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LGC scientist provides keynote at major mass spectrometry conference

15 May 2015
Heidi Goenaga Infante, Principal Scientist and Team Leader of inorganic analysis, has been invited to be part of the scientific committee and to give a keynote lecture on the quantification of metalloproteins relevant to healthcare at the 6th Asia-Pacific Winter Conference on Plasma Spectrochemistry conference in Xiamen, China.
The conference will take place from 19-22 May and aims “to bring together spectroscopists on a worldwide scale to stimulate contacts and exchange of experiences” where world-famous scientists give plenary and keynote lectures.
Heidi’s lecture, ‘Complementary field- flow-fractionation (FFF) and monolithic chromatography with MS detection: A metrological platform for the quantification of metalloproteins relevant to healthcare’, will outline work carried out by LGC in its role as UK designated National Measurement Institute for chemical and bio-measurement. The work was carried out with co-author Raquel Larios of the inorganic analysis team for the European Metrology Research Project HTL05 “Metrology for Metalloproteins”.
The accurate determination of metalloproteins and metal-protein complexes is crucial in clinical and biomedical analysis, as they are increasingly being used as important biomarkers for diseases, for the control of the efficacy of treatments and in drug development. Plasma metal-protein complexes, and in particular metallo-complexes with transferrin and albumin, are the subject of many bio-clinical applications due to the significant role of these proteins in storing and transporting metal ions in this biological fluid. Of particular importance is their involvement in cancer treatment; adducts of plasma proteins (e.g. transferrin, albumin and gammaglobulin) with platinum drugs have been found to be main competitors for the drug binding to DNA in cancer models.
This lecture will discuss the potential of the complementary use of FFF and monolithic chromatography with ICP-MS and ESI MS/MS for the detection, structural confirmation and accurate quantification of adducts of carboplatin with plasma proteins.
Heidi will explain how LGC has developed and validated the first reference methodology based on the use of species-specific isotope dilution calibration. FFF was found to be particularly useful to provide detailed information on the formation of different adduct isoforms during optimisation of conditions for calibrant and spike production (e.g. by incubation of the individual proteins with either, natural or 194Pt-enriched carboplatin).
The lecture will also discuss the main contributing factors to the overall measurement uncertainty and the importance of this work for the provision of reference values to human cancer trials.
For more information visit the conference website.