Cookies on the
LGC website
We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we’ll assume that you are happy to receive all cookies on the LGC website. However, if you would like to, you can change your cookie settings at anytime.
Question markFind out more

KASP genotyping chemistry

KASP™ is our proprietary genotyping technology.

The KASP genotyping reaction comprises three components:

KASP Master mix and KASP Assay mix are used in-house for our genotyping service projects but can also be purchased to run KASP genotyping reactions in your own laboratory.

KASP technology

KASP Assay mix

The KASP Assay mix is specific to the SNP or InDel to be targeted and consists of two competitive, allele-specific forward primers and one common reverse primer. Each forward primer incorporates an additional tail sequence that corresponds with one of two universal FRET (fluorescent resonance energy transfer) cassettes present in the KASP Master mix. All custom KASP assays are available with the additional assay validation service.

KASP Master mix

KASP Master mix contains the two universal FRET cassettes (FAM and HEX), ROX™ passive reference dye, Taq polymerase, free nucleotides and MgCl2 in an optimised buffer solution.


 

Make an enquiry Genotyping

Genotyping prices and ordering

Assay Search Tools

Related downloads

file
KASP genotyping technology 408KB

Brochure

file
Species tested with KASP 156KB

Organisms genotyped using KASP

file
KASP quick start guide 264KB

Outline protocol for set up of KASP genotyping reactions

file
KASP genotyping chemistry 457KB

Full user guide for running KASP in your own laboratory

file
A guide to the analysis of KASP genotyping data using cluster plots 1.1 MB

Cluster plot analysis