MARS (marker assisted recurrent selection) and MABC (marker-assisted backcrossing) breeding programs
LGC specialises in the utilisation of validated KASP™ assays for the development of novel varieties for desirable QTL traits and disease resistance in MARS and MABC programs.
Assay numbers can be small (5-10) and tested on initial multiplication lines for confirmation (10 -30).
Backcrossing has been a widely used technique in plant breeding. Backcrossing is most commonly used to incorporate one or a few genes into an adapted or elite variety. In most cases, the parent used for backcrossing has a large number of desirable attributes but is deficient in only a few characteristics.
With the use of markers, recurrent selection can be accelerated considerably.
MARS and MABC breeding programs in action
We enable our customers to perform a variety of research applications. In one particular study, the International Institute of Tropical Agriculture required appropriate genomic tools to dissect the genetic architecture of economically important traits of Cassava in order to facilitate genetic improvement and enhance marker assisted breeding.
The recent availability of SNP markers coupled with the development of high-precision genotyping technologies and decreasing costs made the utilisation of SNP markers in cassava breeding a realistic goal. The IITA implemented KASP to investigate a subset of 600 informative SNPs to genotype an additional set of 70 F1 progenies.
Using the information generated from KASP, the researchers were able to produce consistent mapping results for most markers, with linkage groups being largely collinear; something that is not normally achieved using other genotyping technologies.
To learn more please register here to receive the full case study and be kept informed of the latest developments register for updates and receive the latest case studies and application notes. This is just one example of how KASP chemistry can help advance MAS research.