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BHQ Probe Master Mix

BHQ® Probe Master Mix is a versatile 2X PCR master mix specially designed to deliver optimal SNP genotyping results for high-throughput, ultra low volume, endpoint probe-based SNP genotyping. BHQ Probe Master Mix frees you to use an extraction method of your choice across a range of probe, sample, and template types. For high-precision genotyping, pair BHQ Probe Master Mix with BHQ or BHQplus® probes for a high-fidelity, low background SNP genotyping assay that yields clear results minus the challenges associated with ambiguous data interpretation.

Benefits of BHQ Probe Master Mix

  • Ultimate versatility - pair with an extraction method and throughput option of your choice and achieve accurate results even in the presence of sample inhibitors in total reaction volumes as low as 0.8 μL.
  • Optimized to pair with BHQplus probes for high-precision, worry-free genotyping
  • Proven reliable and repeatable performance across a range of of challenging plant and animal sample and template types, including AT-rich species

BHQ Probe Master Mix

BHQ Probe Master Mix SNP clusters

Competing master mix

Competing master mix

Figure 1: A direct comparison of BHQ Probe Master Mix to that of a leading market competitor on crudely extracted and challenging sunflower seed samples known to contain inhibitors. BHQ Probe Master Mix shows tight and easily resolvable clusters with limited no template control (NTC) movement even in the presence of sample inhibitors present in higher concentrations due to the very low total reaction volume of 0.8 μL. Competing mix shows incomplete amplification due to inhibitors resulting in samples where genotype cannot be assigned (pink circles). Experiments adhered to respective mix protocols on hot shot extracted sunflower seed samples using BHQplus Probes on a Nexar® Integrated Liquid Handling and Assay Processing System.

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Please choose the correct ROX level for your instrument.

Part Number Description Size
KBS-1040-001 2X BHQ Probe Master Mix, No ROX 1.25 mL
KBS-1040-002 2X BHQ Probe Master Mix, No ROX 2.5 mL
KBS-1040-003 2X BHQ Probe Master Mix, No ROX 5 mL
KBS-1040-004 2X BHQ Probe Master Mix, No ROX 10 mL
KBS-1040-005 2X BHQ Probe Master Mix, No ROX 25 mL
KBS-1040-006 2X BHQ Probe Master Mix, No ROX 200 mL
KBS-1040-011 2X BHQ Probe Master Mix, Low ROX 1.25 mL
KBS-1040-012 2X BHQ Probe Master Mix, Low ROX 2.5 mL
KBS-1040-013 2X BHQ Probe Master Mix, Low ROX 5 mL
KBS-1040-014 2X BHQ Probe Master Mix, Low ROX 10 mL
KBS-1040-015 2X BHQ Probe Master Mix, Low ROX 25 mL
KBS-1040-016 2X BHQ Probe Master Mix, Low ROX 200 mL
KBS-1040-021 2X BHQ Probe Master Mix, Standard ROX 1.25 mL
KBS-1040-022 2X BHQ Probe Master Mix, Standard ROX 2.5 mL
KBS-1040-023 2X BHQ Probe Master Mix, Standard ROX 5 mL
KBS-1040-024 2X BHQ Probe Master Mix, Standard ROX 10 mL
KBS-1040-025 2X BHQ Probe Master Mix, Standard ROX 25 mL
KBS-1040-026 2X BHQ Probe Master Mix, Standard ROX 200 mL