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KASP Master mix

Patented, PCR based KASP genotyping technology has been a critical tool for plant and livestock breeders for the last fifteen years, and the ability to screen KASP markers has underpinned breeding programs at industry leading AgBio companies. In the human genotyping world, KASP markers have been used in large cohort research studies to understand human health and disease.

As the AgBio industry moves towards looking at larger numbers of markers in different plant and animal species, and human health research learns more about the genetics underpinning common diseases, scientists at LGC continue to develop improved genotyping tools compatible with KASP technology.

We use KASP Master mix in our own, largest genotyping lab in Europe, so we can troubleshoot any KASP issues in house with our experts using the same instruments as our customers, and  bring assays in house at times of peak demand or customer laboratory failures.

Key features of KASP master mix supply and production:

  • Each batch of KASP Master mix is functionally tested in high throughput genotyping
  • Our products are patent protected, and have undergone continuous development to incorporate customer performance feedback to arrive at the best ever KASP formulation
  • Our stable supply chain includes disaster recovery processes, allowing us to reliably supply large volumes of KASP Master mix to large, commercial customers
  • Global teams of sales, customer service and technical support specialists, provide regional support for your product
  • Our complete workflow solutions allow us to offer instruments, software, and upstream and downstream technologies that are developed with KASP in mind, with fully validated protocols
  • Order KASP-by-Design (KBD) assays to perform your own validation or KASP-on-Demand (KOD) for fully validated assays delivered by our in-house laboratory

Technical specifications

KASP Master mix contains the universal FRET cassettes, ROX™ passive reference dye, Taq polymerase, free nucleotides and MgCl2 in an optimised buffer solution. The KASP Master mix is universal and can be used in conjunction with all KASP Assays. LGC manufacture KASP Master mix with varying levels of ROX as the qPCR machines available on the market have differing requirements for ROX. KASP Master mix is available in Low, Standard, and High ROX formulations.

Please note: these formulations only differ in the level of ROX that they contain and are otherwise identical. Please refer to the KASP Master mix – PCR instrument guide to select the correct KASP Master mix for your instrument.  If you are using 1536-well plates, please be aware that you will require our 1536-formulation of KASP Master mix (standard ROX only).

Ordering

KASP Master mix can be ordered independently or in conjunction with your KASP assay. Please request a quotation or enquire about pricing, stating the instrument that you intend to perform your genotyping reactions on. The technical support team can then ensure that you purchase the optimal version of KASP Master mix for your qPCR machine. Should you have any questions at all, please contact our customer services team or our technical support team who will be pleased to help you.

Catalogue number Description ROX level Unit size Number of reaction Format
KBS-1016-001 KASP V4.0 2x Master mix Standard 2.5mL 500x10 µL reactions 96/384
KBS-1016-002 KASP V4.0 2X Master mix  Standard 25mL 5,000x10 µL reactions  96/384
KBS-1016-003 KASP V4.0 2X Master mix Standard 250mL 50,000x10 µL reactions 96/384
KBS-1016-010 KASP V4.0 2X Master mix Standard 2.5mL 5,000x1 µL reactions 1536
KBS-1016-011 KASP V4.0 2X Master mix Standard 25mL 50,000x1 µL reactions 1536
KBS-1016-012 KASP V4.0 2X Master mix Standard 250mL 500,000x1 µL reactions 1536
KBS-1016-016 KASP V4.0 2X Master mix Low 2.5mL 500x10 µL reactions 96/384
KBS-1016-017 KASP V4.0 2X Master mix Low 25mL 5,000x10 µL reactions  96/384
KBS-1016-018 KASP V4.0 2X Master mix Low 250mL 50,000x10 µL reactions 96/384
KBS-1016-021 KASP V4.0 2X Master mix High 2.5mL 500x10 µL reactions 96/384
KBS-1016-022 KASP V4.0 2X Master mix High 25mL 5,000x10 µL reactions 96/384
KBS-1016-023 KASP V4.0 2X Master mix High 250mL 50,000x10 µL reactions 96/384
KBS-1030-001 KASP Array Tape 2X Master mix Standard 2.5mL See Manual Array Tape
KBS-1030-002 KASP Array Tape 2X Master mix Standard 25mL See Manual Array Tape
KBS-1030-003 KASP Array Tape 2X Master mix Standard 250mL See Manual Array Tape
 

For research use only. Not for use in diagnostic procedures.