KASP™-TF Master Mixes are formulated without Triton™ X-100. In addition to our range of standard KASP genotyping reagents, we provide a specialised KASP-TF formulation specifically for use with the specialist optics and reduced sample volumes used in the Fluidigm genotyping system. A validation service is available for all KASP assays used with the Fluidigm system. Please note: KASP-TF reagents formulated for the Fluidigm system are not interchangable with our standard KASP-TF reagents.
The small size of the individual reaction chambers within the Fluidigm chips leads to the requirement for a high starting concentration of DNA. For human DNA, the required starting concentration is 60 ng / µL; this figure should be proportionally adjusted based on the genome size of the organism of interest. Where DNA concentration is not sufficient, it is imperative to amplify the specific SNP-containing regions from the template DNA prior to performing genotyping on the Fluidigm system. The technique of pre-amplification can be used to amplify the regions of interest by designing primers specific to these regions, and can be multiplexed. The resultant amplicon mixture can then be diluted and used as template for KASP genotyping on the Fluidigm system.
LGC can supply primers for the pre-amplification reaction in conjunction with each KASP Assay ordered. Please specify that you require pre-amplification primers when placing your order.
If you have any queries about running KASP on the Fluidigm system or performing pre-amplification reactions, please do not hesitate to contact our technical support team.
| Catalogue number | Description |
|---|---|
| KBS-1050-145 |
KASP-TF for Fluidigm (400 µL) |
| KBS-1050-146 | KASP-TF for Fluidigm (4 mL) |
Ordering
Review our KASP Reagents Guidance Notes for step-by-step guide to ordering KASP reagents and delivery information, or request a quote or enquire about pricing, noting that your requirement is for KASP-TF reagents for Fluidigm in the comments section.