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Rice genotyping library

A set of 2055 KASP™ SNP genotyping assays for rice has been developed for the Generation Challenge Programme and their Integrated Breeding Platform. Through LGC, these assays are now available to the rice breeding community to accelerate development of improved varieties by marker-assisted selection (MAS).

The rice genotyping library utilises our accurate and robust PCR-based KASP genotyping chemistry. Bespoke sets of assays can be conveniently selected with the Assay Search Tool.

 
Unlike chip-based systems with fixed marker sets, our rice genotyping library allows you to flexibly pick and choose sets of markers according to changing needs arising in marker-assisted breeding projects. Furthermore, KASP genotyping assays are highly cost-effective, thus providing an economical option to use the same SNP assays throughout the course of a breeding programme.
 
LGC’s complete genotyping offering also encompasses plant sample collection kits, DNA extraction and genotyping services, enabling breeders even in remote locations to apply MAS.


Development of the rice genotyping library

The conversion of rice SNPs to KASP assays was commissioned by the Generation Challenge Programme to produce a set of markers polymorphic within and between the main Oryza groups. A subset of these markers has been used to validate Oryza glaberrima/O. sativa polymorphisms (Pariasca-Tanaka et al., 2014).


Advantages

  • Library includes 2055 functionally validated SNP markers
  • Cost-effective and robust KASP genotyping technology
  • Flexible - pick and choose the markers that are relevant to your project based on genome location
  • Convenient – use informative markers directly on breeding populations or novel cultivars
  • No minimum order – order any number of assays from the library
  • KASP assays are easy to run in your own laboratory or can be used in a genotyping service project


Ordering

The rice KASP Assays can be provided as part of our genotyping service or as reagents to run KASP genotyping in your own laboratory.

With our Assay Search Tool, you can search for specific assays, export your selection, pick the required amount of KASP Master mix, and request a quote. To place an order straightaway for all assays in your cart, please use the “export assay cart” function and forward the exported Excel file together with your purchase order to orders.uk@lgcgroup.com
 
The assays are available in 2 pack sizes:
Cat. No Product Pack size
KBS-1023-042 Rice KASP Assay Mix 2500 x 10 μL reactions
KBS-1023-043 Rice KASP Assay Mix 6000 x 10 μL reactions
For enquiries about a genotyping service project, please request a quotation or contact our customer services team.


References

Integrated Breeding Platform (IBP):
https://www.integratedbreeding.net
 
Development of genome-wide SNP assays for rice. McCouch, S.R., Zhao, K., Wright, M., Tung C.W., Ebana, K., Thomson, M., Reynolds, A., Wang, D., DeClerck, G., Ali, L., McCIung, A., Eizenga, G., Bustamante, C. (2010). Breeding Science 60: 524–535.

Development of a SNP genotyping panel for detecting polymorphisms in Oryza glaberrima/O. sativa interspecific crosses. Pariasca-Tanaka, J., Lorieux, M., He, C., McCouch, S., Thomson, M.J., Wissuwa, M. (2014). Euphytica 201(1): 67-78.