DNA/RNA shearing instruments for NGS

In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. To enable sensitive and reliable NGS results DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform).

To supply you with the best tools, support and quality products for your NGS projects, we've partnered with Diagenode to distribute their range of shearing instruments.* Diagenode has developed the most advanced water bath sonicators (the Bioruptor^® for 150 bp – 2 kb), a unique desktop sonication device (Diagenode One^® for 150 bp – 1 kb) and a unique hydrodynamic shearing instrument (the Megaruptor^® for 2 kb – 75 kb). Please view the chart below for a quick overview and comparison of these instrument options

Compare your options

Options	Bioruptor Pico Advanced water bath sonicator	Bioruptor Plus Advanced water bath sonicator	Megaruptor 2 Hydrodynamic shearing instrument	Diagenode One Desktop sonication device
Recommended use	ChIP-seq and NGS sample preparation	Cell and tissue sample preparation	Ultimate solution for long read sequencing library preparation	Compact bench top device for simple NGS library preparation
Size	35.5 [W] x 24.5 [D] x 28 [H] cm 14 [W] x 9.6 [D] x 11 [H] in 7.9 kg (17.4 lb)	Control unit dimensions: 35 [W] x 26 [D] x 16.5 [H] cm 13.8 [W] x 10.2 [D] x 6.5 [H] in Soundproof box dimensions: 35 [W] x 35 [D] x 26.5 [H] cm 13.8 [W] x 13.8 [D] x 10.4 [H] in 30 kg (66.1 lb)	20 [W] x 23 [D] x 39.5 [H] cm 7.9 [W] x 9.1 [D] x 15.6 [H] in 8.2 kg (18.1 lb)	20 [W] x 28.5 [D] x 16.5 [H] cm 7.9 [W] x 11.2 [D] x 6.5 [H] in 5 kg (11 lb)
Key applications	Chromatin shearing 200 bp – 1 kb DNA shearing 150 bp – 1 kb RNA shearing 200 bp – 1 kb FFPE nucleic acid extraction Cell lysis and tissue disruption	Chromatin shearing 200 bp – 1 kb DNA/RNA/Protein extraction Mass spectrometry Chemical applications	DNA Shearing 3 kb - 75 kb (2 - 9 kb Hydropore Short and 10 - 75 kb Hydropore Long)	DNA shearing 200 bp – 1 kb Chromatin shearing 200 bp – 1 kb
Throughput	16 samples (0.2 ml tube holder) 12 samples (0.1 and 0.65 ml tube holders) 6 samples (1.5 and 15 ml tube holders)	12 (0.5 ml tube holder) 6 (1.5 ml tube holder) 6 (15 ml tube holder) 3 (50 ml tube holder)	2 samples (process in series)	1 sample
Volumes	5 - 50 µl (0.1 ml tubes) 20 - 100 µl (0.2 ml tubes) 100 µl (0.65 ml tubes) 100 - 300 µl (1.5 ml tubes) 300 µl - 2 ml (15 ml tubes)	100 μl (0.5 ml Bioruptor tubes) 100 - 300 μl (1.5 ml Bioruptor tubes) 300 μl - 2 ml (15 ml Bioruptor tubes) 2 - 20 ml (50 ml tubes)	50 - 400 µl (hydrotubes) Recommended: 200 µl	20 and 50 µl
Additional highlights	Temperature controlled Digital timer	Temperature controlled	Temperature controlled Touchscreen with Megaruptor software	Temperature controlled
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Need further guidance on which shearing instrument is right for you? Contact our team for a free assessment.

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* LGC’s distribution agreement includes EMEA, South America and Canada for all market sectors and the United States for the agricultural biotechnology sector.