DNA/RNA shearing instruments for NGS
In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. To enable sensitive and reliable NGS results DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform).
To supply you with the best tools, support and quality products for your NGS projects, we've partnered with Diagenode to distribute their range of shearing instruments.* Diagenode has developed the most advanced water bath sonicators (the Bioruptor® for 150 bp – 2 kb), a unique desktop sonication device (Diagenode One® for 150 bp – 1 kb) and a unique hydrodynamic shearing instrument (the Megaruptor® for 2 kb – 75 kb). Please view the chart below for a quick overview and comparison of these instrument options
Compare your options
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Options |
Bioruptor Pico
Advanced water bath sonicator
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Bioruptor Plus
Advanced water bath sonicator
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Megaruptor 2
Hydrodynamic shearing instrument
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Diagenode One
Desktop sonication device
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Recommended use |
ChIP-seq and NGS sample preparation |
Cell and tissue sample preparation |
Ultimate solution for long read sequencing library preparation |
Compact bench top device for simple NGS library preparation |
Size |
35.5 [W] x 24.5 [D] x 28 [H] cm
14 [W] x 9.6 [D] x 11 [H] in
7.9 kg (17.4 lb) |
Control unit dimensions:
35 [W] x 26 [D] x 16.5 [H] cm
13.8 [W] x 10.2 [D] x 6.5 [H] in
Soundproof box dimensions:
35 [W] x 35 [D] x 26.5 [H] cm
13.8 [W] x 13.8 [D] x 10.4 [H] in
30 kg (66.1 lb) |
20 [W] x 23 [D] x 39.5 [H] cm
7.9 [W] x 9.1 [D] x 15.6 [H] in
8.2 kg (18.1 lb) |
20 [W] x 28.5 [D] x 16.5 [H] cm
7.9 [W] x 11.2 [D] x 6.5 [H] in
5 kg (11 lb) |
Key applications |
Chromatin shearing 200 bp – 1 kb
DNA shearing 150 bp – 1 kb
RNA shearing 200 bp – 1 kb
FFPE nucleic acid extraction
Cell lysis and tissue disruption |
Chromatin shearing 200 bp – 1 kb
DNA/RNA/Protein extraction
Mass spectrometry
Chemical applications |
DNA Shearing 3 kb - 75 kb
(2 - 9 kb Hydropore Short and 10 - 75 kb Hydropore Long) |
DNA shearing 200 bp – 1 kb
Chromatin shearing 200 bp – 1 kb |
Throughput |
16 samples (0.2 ml tube holder)
12 samples (0.1 and 0.65 ml tube holders)
6 samples (1.5 and 15 ml tube holders) |
12 (0.5 ml tube holder)
6 (1.5 ml tube holder)
6 (15 ml tube holder)
3 (50 ml tube holder) |
2 samples (process in series) |
1 sample |
Volumes |
5 - 50 µl (0.1 ml tubes)
20 - 100 µl (0.2 ml tubes)
100 µl (0.65 ml tubes)
100 - 300 µl (1.5 ml tubes)
300 µl - 2 ml (15 ml tubes) |
100 μl (0.5 ml Bioruptor tubes)
100 - 300 μl (1.5 ml Bioruptor tubes)
300 μl - 2 ml (15 ml Bioruptor tubes)
2 - 20 ml (50 ml tubes) |
50 - 400 µl (hydrotubes)
Recommended: 200 µl |
20 and 50 µl |
Additional highlights |
Temperature controlled
Digital timer |
Temperature controlled |
Temperature controlled
Touchscreen with Megaruptor software |
Temperature controlled |
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Request a quote
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Need further guidance on which shearing instrument is right for you? Contact our team for a free assessment.
Library prep automation with IP-Star® Compact
Automate: End repair -> Clean-up -> Adenylation -> Adapter ligation -> Stop ligation -> 2x Clean-up -> Bead size selection
- Quick and easy set up
- 15 minutes hands-on time
- Compatible with multiplexing protocols
- Process 1 to 16 samples per run with standard protocols (32/days)
* LGC’s distribution agreement includes EMEA, South America and Canada for all market sectors and the United States for the agricultural biotechnology sector.