Good practice guide for the application of quantitative PCR
To downlad a copy of the 'Good practice guide for the application of quantitative PCR (qPCR)', click on the image on the right.
This guide is aimed at individuals who are starting to use qPCR and realise that, while this method is easy to perform in the laboratory, numerous factors must be considered to ensure that the method will be applied correctly.
The guide aims to assist those who are, or will be, using qPCR by discussing the issues that need consideration during experimental design. The guide includes “tried and tested” approaches, and troubleshoots common issues.
This guide was produced with support from the UK National Measurement System.
Authors: T Nolan, J Huggett, E Sanchez
Published: 11 October 2013
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a copy of the guide.