DNA extraction instrumentation

Significant advances in plant breeding have been facilitated by the rapid and continued advancement of molecular biology-based technologies. These are driving the need for new automated extraction platforms that combine higher sample throughputs with the delivery of highly purified DNA that is suitable for a wide range of downstream applications.
The oKtopure™ robot has been developed to deliver automated DNA extraction from 8 x 96 well microtitre plates in parallel; utilising the proprietary sbeadex magnetic bead based chemistry. The instrument delivers standardised, medium-throughput extractions and results in purification of high quality DNA suitable for all molecular biology based downstream applications including sequencing (NGS, Sanger) and SNP genotyping (e.g. KASP™).
The Genespin™ instrument delivers a higher sample throughput and is optimised for Kleargene, our spin plate-based DNA extraction chemistry. The instrument delivers standardised, semi-automated DNA extractions for downstream PCR-based applications. The instrument is compatible with both 96- and 384-well spin plates, and can be used to perform all lysate transfer and buffer addition steps.
Key feature |
oKtopure
|
Genespin
|
Compatible chemistry
|
sbeadex™
|
Kleargene
|
Extraction technology
|
Surface-modified superparamagnetic particles
|
Spin plates: glass fibre solid support inserted to microtitre plate
|
Plate format
|
96-well
|
96-well or 384-well |
Grade of automation
|
Fully automated |
Semi automated
|
Throughput
|
up to 3.000 - 4.000 samples / day
|
up to 15.000 - 20.000 samples / day
|
DNA quality
|
Very high
|
Very good
|
Instrument costs
|
Please enquire
|
Please enquire
|
Consumables costs
|
low
|
very low
|
Sample type
|
Leaf and seed tissue
|
Leaf and seed tissue
|
Downstream applications
|
All genomics based applications including Next-generation sequencing, Sanger sequencing, SNP genotyping
|
PCR based applications
|