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LGC Standards helps laboratories to shed light on global vitamin D deficiency

04 Aug 2010
Tags:  Standards

As global concerns about vitamin D deficiency increase, LGC Standards launches range of reference materials for laboratories monitoring vitamin D

With vitamin D deficiency being implicated in an increasing number of medical conditions, LGC Standards, Europe’s leading supplier of reference materials, has launched a range of reference materials for laboratories monitoring vitamin D levels.

Global research is revealing the importance of vitamin D in protecting against a host of health problems such as rickets, cardiovascular disease, various cancers, multiple sclerosis, bone diseases, respiratory infection and depression. It has been suggested that approximately 1 billion people around the world may suffer from some form of vitamin D deficiency. Formation of vitamin D occurs within the body and requires exposure to sunlight. The increasing popularity of indoor activities such as computer gaming, combined with public health policy encouraging people to reduce their exposure to sunlight to reduce the risk of skin cancer may be contributing to the risk of vitamin D deficiency.

The key analytical target compounds for vitamin D monitoring have been identified as hydroxy and dihydroxy metabolites of vitamin D. Monitoring of these key metabolites is now routine, with laboratories using both immunoassay and liquid chromatography–mass spectrometry and its variants (LC-MSn). The growing availability of LC-MS/MS has permitted the development of assays which can specifically target the required analytes with the greatest clinical significance.

LGC Standards has therefore brought together a range of reference materials to satisfy laboratories’ requirements for instrumental analysis of vitamin D-related substances. These reference materials, from matrix-matched serum samples to deuterium labelled internal standards, provide laboratories with the reference standards they require to deliver accurate and reliable results to clinicians and their patients.

The complete range of vitamin D reference materials available from LGC Standards includes:

Matrix matched materials

NIST-972                      vitamin D metabolites in human serum


NIST-2972                    25-Hydroxyvitamin D2 and D3 calibration solutions
CERH-073                    25-Hydroxyvitamin D2 (50ug/ml)
CERH-087                    25-Hydroxyvitamin D2 (5 µg/ml)
CERH-083                    25-Hydroxyvitamin D3 (100 µg/ml)
CERH-086                    5-Hydroxyvitamin D3 (5 µg/ml)

Solid material

TRC-C125700              25-Hydroxyvitamin D3
TRC-H995820              25-Hydroxyvitamin D2
TRC-C144500              1,25-Dihydroxyvitamin D3

Deuterium labelled materials (all D6 labelled)

CERH-074                    25-Hydroxyvitamin D3
TRC-C125702              25-Hydroxyvitamin D3
TRC-C144502              1,25-Dihydroxyvitamin D3

These reference materials are available from LGC Standards (, the division of LGC that provides products and services to support measurement in the laboratory. For prices and ordering information, please email: or tel: +44 (0)20 8943 8480.