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The Jackson Laboratory – Mouse genomics app note

Trans-regulation of mouse meiotic recombination hotspots by Rcr1

Meiotic homologous recombination generates genetic variety amongst offspring, and ensures that the chromosomes are segregated accurately during meiosis. Recombination occurs in hotspots, and the aim of this study was to identify trans-acting factors that control hotspot positioning in mammals, specifically congenic mice, using a combination of analyses including SNP genotyping.

Use of KASP to develop speed congenics

Congenic strains of mice typically require 10 generations of backcrossing to generate, which can be a time-consuming process. The time involved in creating congenic mice can be significantly reduced through the use of marker-assisted selection (MAS), and this is termed ‘speed congenics’. Genetic markers, such as SNPs are used to identify mice from the second generation of backcrossing that contain the highest percentages of the desired genome; these mice are then used as parents for the subsequent generation. This marker-assisted selection procedure is repeated for each subsequent generation, and hence enables congenic mice to be attained after only three or four generations of backcrossing. Read here about our ‘mouse panel’ which offers a set of pre-validated KASP genotyping assays that can be easily applied to the development of speed congenics.

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Jackson Laboratory – mammalian genetics research to enhance human health 929KB

Application note: Trans-regulation of mouse meiotic recombination hotspots by Rcr1.

Related links

Link Mouse panel