KASP genotyping assay
The patented PCR-based KASP™ genotyping assay is a homogeneous, fluorescence (FRET) based assay that enables accurate bi-allelic discrimination of known SNPs and InDels. Unlike other PCR based genotyping assays, KASP requires no labelling of the target-specific primers / probes, giving it a clear cost advantage. It also allows greater flexibility for assay design, which translates into a higher overall success rate and the unique ability to genotype large / mega InDels.
Why use KASP?
The simplicity of the KASP assay design yields clear end-user advantages including:
Superb accuracy and performance
Accuracy: >99.8% based on independent assessment
Industry leading SNP & InDel assay conversion rate (>90%)
Flexible primer design which increases the rate of successful assay development
Supports low-, medium- and high-throughput studies and individual repeat assays
Compatibility with a range of liquid handling systems and thermal cyclers, signal can be read on most FRET-capable plate readers and qPCR instruments.
Breakthrough cost savings
KASP uses a universal reporting system where the labelled components are present in the KASP Master mix
Eliminates the need for expensive labelled assay-specific primers or probes
Requires only 10 ng DNA per sample per SNP (based on human genome size)
Low reaction volumes keep reagent costs to a minimum
Cost benefits enable you to perform more assays overall, improving the quality of your data.
The KASP genotyping assay was developed for use in our own service labs where it has been in use for over 10 years. Our KASP end-point PCR technology has been referenced in over 2,000 publications.
Choose KASP to purchase as a product (KBD - KASP by Design) for your lab or as a genotyping service.
A key feature of our PCR-based KASP technology is the use of a universal FRET cassette reporter system that eliminates the need for costly dual-labelled probes. Our allele-specific forward primers each have a proprietary tail sequence that corresponds with one of two FRET cassettes: one label with FAM dye and the other with HEX dye. This technology gives KASP a clear cost advantage, and when combined with miniaturised reaction volumes (10 µL in 96-well PCR plates, 5 µL in 384-well PCR plates; 1 µL in 1536-well PCR plates), delivers an exceptionally accurate, flexible, and cost-effective genotyping solution. Bi-allelic discrimination is achieved through the competitive binding of the two allele-specific forward primers.
No additional equipment
The KASP genotyping technology is based on an open format PCR workflow. There is no need to buy new expensive equipment to be able to run KASP in your own lab; completed KASP reactions can be read on most FRET-capable plate readers and qPCR instruments.