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KASP technical resources

Below you will find a comprehensive list of all our documents related to genotyping with a link to download.

Title Description  
KASP manual Full user guide for running KASP in your own laboratory Download
KASP quick start guide Outline protocol for set up of KASP genotyping reactions Download 
How does KASP work? Overview of how KASP genotyping chemistry works and how data is scored at LGC Download
How much DNA should I send for my genotyping project? This document outlines the amount of DNA required for a genotyping project run in our service laboratories Download
Reference list Partial list of species that have been genotyped using KASP Download
Trial kit manual User guide for running the KASP genotyping trial kit Download
Trial kit quick start guide Outline protocol for running the KASP genotyping trial kit Download
Validation kit manual User guide for running the KASP Genotyping validation kit (only sent to customers that are using instruments that we are not familiar with). Download
Running KASP on the ABI 7500 User manual for setting up and running KASP on the ABI 7500 qPCR instrument Download
Running KASP on the ABI 7900 User manual for setting up and running KASP on the ABI 7900 qPCR instrument Download
Running KASP on the ABI StepOne and StepOne Plus User manual for setting up and running KASP on the ABI StepOne and StepOne Plus qPCR instrument Download
Running KASP on the ABI Viia7 User manual for setting up and running KASP on the ABI ABI Viia7 qPCR instrument Download
Running KASP on the BIO-RAD CFX User manual for setting up and running KASP on the BIO-RAD CFX qPCR instrument Download
Running KASP on the Roche LC480 User manual for setting up and running KASP on the Roche LC480 qPCR instrument Download
Running KASP on the illumina EcoRT User manual for setting up and running KASP on the illumina EcoRT qPCR instrument Download
Running KASP on the Agilent Mx3000 / Mx3005P User manual for setting up and running KASP on the Agilent Mx3000 / Mx3005P instrument Download
Running KASP on Roche LC96 Factsheet gives detailed explanation of the issues
running KASP genotyping on an LC96 instrument
Download
KASP thermal cycling conditions A document containing details of all the commonly used thermal cycling protocols for KASP genotyping. Download
KASP thermal cycling 61-55ºC touchdown protocol The standard KASP thermal cycling conditions.   Download
KASP thermal cycling 68-62ºC touchdown protocol An alternative KASP thermal cycling protocol, typically used for assays with high %GC. Download
KASP thermal cycling 2-step 57ºC protocol An alternative KASP thermal cycling protocol, typically used for assays with low %GC. Download
KASP thermal cycling 65-57ºC touchdown protocol The standard KASP thermal cycling conditions for the Fluidigm platform. Download
KASP thermal cycling recycling protocol The thermal cycling conditions for further cycling (‘recycling’) of KASP genotyping reactions. Download
Running KASP on Thermo Scientific Piko Real User manual for setting up and running KASP on the Thermo Scientific PikoReal instrument Download