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Supercharging reagents - revving up peptide LC-MS analyses

  • Date:
  • 11 Nov 2016

Over the past 5 years, LC-MS has (arguably) become the technique of choice to quantify peptides in biological matrices. The benefits of using LC-MS as a quantitative technique are significant, however, there is one drawback – low sensitivity. The acquisition of multiple numbers of charge states for peptides in the MS source can result in significant sensitivity loss, as usually only a single charge state is selected for quantitation. Modifying the ionisation characteristics of peptides in the source is difficult, and changing the level of normal mobile phase modifiers has minimal effects. The addition of supercharging reagents to mobile phases significantly shifts the charge states of peptides and proteins present in the ESI source. This enables the analyst to change the selected precursor for quantitative purposes, with potential sensitivity gains. Sensitivity can be increased if a higher charge state gives a better fragmentation profile. Selectivity can also be improved, where selecting a different precursor removes signal from co-eluting interferents. A number of examples of the application of supercharging reagents to peptide LC-MS analyses will be described, and the impact on the quantitative performance discussed.

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Why mobile security matters

  • Date:
  • 25 Aug 2016
  • Duration:
  • 2:30-3:00 BST - 30-45 minutes

The exponential development of technology in the last 10 years has allowed the relative layman to seize and utilise tools which were once only accessible by the technically adept.

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In Vitro Antimicrobial Efficacy Testing: Potential Pitfalls and Future Methods

  • Date:
  • 04 May 2016
  • Duration:
  • 30 Minutes 2.30 - 3:00 pm GMT

Join us for our webinar on in vitro antimicrobial afficacy testing: potential pitfalls and future methods

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Quantitation of low level impurities using LC-MS/MS in GMP analysis

  • Date:
  • 25 Feb 2016
  • Duration:
  • 30-45 Minutes, 2:30-3:15pm GMT

LC-MS/MS has been the technique of choice for trace organic analysis in most areas of analytical chemistry for over a decade and is now slowly but steadily becoming so in GMP pharmaceutical analysis. Join us for our webinar to find out more.

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Cyber Defence and EU General Data Protection Reform - are you ready?

  • Date:
  • 12 Feb 2016
  • Duration:
  • 30-45 Minutes, 2:00-2:45pm GMT

Join this webinar to get an insight to imminent data protection reform from the EU and the importance of forensic readiness as part of cyber breach preparation.

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Little to LARGE: LC-MS/MS peptide method development for small molecule specialists

  • Date:
  • 03 Feb 2016
  • Duration:
  • 30 Minutes 2.30 - 3:00pm GMT

Using glucagon, a notoriously difficult to quantify endogenous hormone, as a model peptide and ibuprofen as a model small molecule, James Howard, Scientist at LGC, will discuss the following during the webinar.

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