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DNA/RNA shearing instruments for NGS

In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. To enable sensitive and reliable NGS results DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform).

To supply you with the best tools, support and quality products for your NGS projects, we've partnered with Diagenode to distribute their range of shearing instruments.* Diagenode has developed the most advanced water bath sonicators (the Bioruptor® for 150 bp – 2 kb), a unique desktop sonication device (Diagenode One® for 150 bp – 1 kb) and a unique hydrodynamic shearing instrument (the Megaruptor® for 2 kb – 75 kb). Please view the chart below for a quick overview and comparison of these instrument options

Compare your options

  Bioruptor Pico Bioruptor Plus Megaruptor 2 Diagenode One
Options

Bioruptor Pico
Advanced water bath sonicator

Bioruptor Plus
Advanced water bath sonicator

Megaruptor 2
Hydrodynamic shearing instrument

Diagenode One
Desktop sonication device

Recomended use ChIP-seq and NGS sample preparation Cell and tissue sample preparation Ultimate solution for long read sequencing library preparation Compact bench top device for simple NGS library preparation
Size 35.5 [W] x 24.5 [D] x 28 [H] cm
14 [W] x 9.6 [D] x 11 [H] in
7.9 kg (17.4 lb)
Control unit dimensions:
35 [W] x 26 [D] x 16.5 [H] cm
13.8 [W] x 10.2 [D] x 6.5 [H] in
Soundproof box dimensions:
35 [W] x 35 [D] x 26.5 [H] cm
13.8 [W] x 13.8 [D] x 10.4 [H] in
30 kg (66.1 lb)
20 [W] x 23 [D] x 39.5 [H] cm
7.9 [W] x 9.1 [D] x 15.6 [H] in
8.2 kg (18.1 lb)
20 [W] x 28.5 [D] x 16.5 [H] cm
7.9 [W] x 11.2 [D] x 6.5 [H] in
5 kg (11 lb)
Key applications Chromatin shearing 200 bp – 1 kb
DNA shearing 150 bp – 1 kb
RNA shearing 200 bp – 1 kb
FFPE nucleic acid extraction
Cell lysis and tissue disruption
Chromatin shearing 200 bp – 1 kb
DNA/RNA/Protein extraction
Mass spectrometry
Chemical applications
DNA Shearing 3 kb - 75 kb
(2 - 9 kb Hydropore Short and 10 - 75 kb Hydropore Long)
DNA shearing 200 bp – 1 kb
Chromatin shearing 200 bp – 1 kb
Throughput 16 samples (0.2 ml tube holder)
12 samples (0.1 and 0.65 ml tube holders)
6 samples (1.5 and 15 ml tube holders)
12 (0.5 ml tube holder)
6 (1.5 ml tube holder)
6 (15 ml tube holder)
3 (50 ml tube holder)
2 samples (process in series) 1 sample
Volumes 5 - 50 µl (0.1 ml tubes)
20 - 100 µl (0.2 ml tubes)
100 µl (0.65 ml tubes)
100 - 300 µl (1.5 ml tubes)
300 µl - 2 ml (15 ml tubes)
100 μl (0.5 ml Bioruptor tubes)
100 - 300 μl (1.5 ml Bioruptor tubes)
300 μl - 2 ml (15 ml Bioruptor tubes)
2 - 20 ml (50 ml tubes)
50 - 400 µl (hydrotubes)
Recommended: 200 µl
20 and 50 µl
Additional highlights Temperature controlled
Digital timer
Temperature controlled Temperature controlled
Touchscreen with Megaruptor software
Temperature controlled
 

Learn more

Learn more

Learn more

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Request a quote

Request a quote

Request a quote

Request a quote

Need further guidance on which shearing instrument is right for you? Contact our team for a free assessment.

Library prep automation with IP-Star® Compact

Automate: End repair -> Clean-up -> Adenylation -> Adapter ligation -> Stop ligation -> 2x Clean-up -> Bead size selection

  • Quick and easy set up
  • 15 minutes hands-on time
  • Compatible with multiplexing protocols
  • Process 1 to 16 samples per run with standard protocols (32/days)

* LGC’s distribution agreement includes EMEA, South America and Canada for all market sectors and the United States for the agricultural biotechnology sector.