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Analysis of KASP genotyping data using cluster plots

A document explaining how to analyse KASP genotyping data using cluster plots, and to provide troubleshooting for common issues that can arise.


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DNA requirements for laboratory-validated KASP assays (KASP-On-Demand)

Requirements for DNA sample submissions, DNA mass and concentration


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Filter set information for KASP on the LC480 vI and vII

Details regarding the filter set options that should be used when running KASP on the LC480 vI and vII instruments.


254 KB

Hydrocycler2 - data sheet

Hydrocycler2 for high throughput PCR


709 KB

KASP genotyping trial kit manual

User guide for running the KASP genotyping trial kit


242 KB

KASP quick start guide

Outline protocol for set up of KASP genotyping reactions


342 KB

KASP thermal cycling 2-step 57ºC protocol

An alternative KASP thermal cycling protocol, typically used for assays with low %GC.


114 KB

KASP thermal cycling 61-55ºC touchdown protocol

The standard KASP thermal cycling conditions.


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KASP thermal cycling 65-57ºC touchdown protocol

The standard KASP thermal cycling conditions for the Fluidigm platform.


119 KB

KASP thermal cycling 68-62ºC touchdown protocol

An alternative KASP thermal cycling protocol, typically used for assays with high %GC.


119 KB

KASP thermal cycling conditions

A document containing details of all the commonly used thermal cycling protocols for KASP genotyping.


206 KB

KASP thermal cycling recycling protocol

The thermal cycling conditions for further cycling (‘recycling’) of KASP genotyping reactions.


112 KB

KASP trial kit quick start guide

Outline protocol for running the KASP genotyping trial kit


128 KB

KASP troubleshooting guide

User guide


851 KB

KASP validation kit manual

Outline protocol for running the KASP genotyping validation kit


276 KB

KlusterCaller and Kraken Plate Data Import User Guide

Guide to importing genotyping data into KlusterCaller / Kraken


2331 KB

Running KASP on Qiagen RotorGene Q

Guide to running KASP genotyping reactions on the QIAGEN Rotor-Gene® Q instrument


2461 KB

Running KASP on the ABI 7500

User manual for setting up and running KASP on the ABI 7500 qPCR instrument


597 KB

Running KASP on the ABI 7900

User manual for setting up and running KASP on the ABI 7900 qPCR instrument


1239 KB

Running KASP on the ABI StepOne and StepOnePlus

User manual for setting up and running KASP on the ABI StepOne and StepOnePlus qPCR instrument


966 KB

Running KASP on the ABI Viia7

User manual for setting up and running KASP on the ABI Viia7 qPCR instrument


1080 KB

Running KASP on the Agilent Mx3000 / Mx3005P

User manual for setting up and running KASP on the Agilent Mx3000 / Mx3005P instrument


747 KB

Running KASP on the BIO-RAD CFX

User manual for setting up and running KASP on the BIO-RAD CFX qPCR instrument


968 KB

Running KASP on the illumina EcoRT

User manual for setting up and running KASP on the illumina EcoRT qPCR instrument


623 KB

Running KASP on the Roche LC480

User manual for setting up and running KASP on the Roche LC480 qPCR instrument


2742 KB

Running KASP on the Roche LC96

Factsheet gives a detailed explanation of the issues when running KASP genotyping on an LC96 instrument and the work-around solutions


546 KB

Running KASP on the Thermo Scientific PikoReal

User manual for setting up and running KASP on the Thermo Scientific PikoReal instrument.


832 KB

SNPviewer manual

User manual for LGC’s SNPviewer software


770 KB