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KlearKall Master mix

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Designed by experts in PCR, KlearKall™ Master mix has been optimised to perform high-quality end-point genotyping on large numbers of samples. On both array tape and plate based genotyping systems, KlearKall performance has been found to be superior or equivalent to competitor mixes, in terms of both cluster separation and amplification efficiency.

Analyse high numbers of samples quickly and accurately

Whether using Douglas Scientific’s Array Tape technology, 384- or 1536-well plates, KlearKall is superior across all formats to deliver discrete clusters and high call rates for accurate and reproducible allelic discrimination with hydrolysis probe-based genotyping, even with difficult assays.

  • Proven accuracy and reliability even on crude samples
  • Low volume reactions (down to 1 µL) – reducing cost and increasing throughput
  • Compatible with hydrolysis probe-based genotyping assays such as BHQ®, BHQplus®, and TaqMan®.
  • Universal thermal cycling conditions for most assays, and across platforms
  • Bench top stability for ease and flexibility of use
  • Excellent value for money
  • Contains KlearTaq™ Hot Start DNA polymerase plus optimised components, and  passive reference dye ROX to enhance precision of data analysis
  • Operates on most qPCR instruments and FRET-capable plate readers

Klearkall vs. competitor mixes on crude DNA lysate samples on Nexar Array Tape
Figure 1:Klearkall vs. competitor mixes on crude DNA lysate samples on Nexar Array Tape (1.6 µL reactions)

Klearkall vs. competitor mixes on crude DNA lysate samples on 384-well plates (3 μL reactions).
Figure 2: Klearkall vs. competitor mixes on crude DNA lysate samples on 384 well plates (3 μL reactions).

Klearkall vs. competitor mixes on crude DNA lysate samples on 1536-well plates (1 μL reactions).
Figure 3: Klearkall vs. competitor mixes on crude DNA lysate samples on 1536-well plates (1 μL reactions).

LGC – a complete solution for your high-throughput genotyping workflow

With the acquisition of BioSearch Technologies, LGC has become a single, unified source for sample preparation, PCR reagents, probes, consumables and instrumentation. With the combined PCR expertise of LGC and Biosearch, we can supply everything you need to run your assays at your own facility. Alternatively, take advantage of our experienced service laboratories for a convenient and efficient service from sample through to actionable data.

Terms and Conditions

  • Offer valid for end users only – not for re-sale.
  • The purchase of this kit to perform the 5’ nuclease assay does not convey a license to any patents that may be in existence for this technology.
  • For research use only. Not for use in diagnostic procedures.
  • Sample pack: 2.5 mL KlearKall Master mix
  • Offer limited to 1 free sample per laboratory
  • LGC terms and conditions apply.